Biosafety Cabinet: one of safety tools in Laboratories

The Biosafety cabinet (BSC) is essential equipment in Laboratories that handle hazardous biological materials. This cabinet provide a controlled environment to protect Laboratory personnel, the environment, and the integrity of experiments conducted within.

The Biosafety cabinet comes in various classes and types, each designed for specific applications and levels of containment. The three primary types are:

Class I:

This cabinet provide personnel protection, suitable for working with low to moderate risk biological agents. Air is drawn into the cabinet, and a portion is exhausted, while the rest is recirculated through a HEPA filter to remove contaminants.

Class II:

Class II cabinet, the most common type, offer both personnel and product protection. They are classified into type A1, A2, B1, and B2, based on the level of protection they provide and their exhaust systems. The Class II cabinet is essential for working with biological agents that require a higher level of containment.

Class III:

Class III cabinet, also known as gloveboxes or gas-tight cabinet, is designed for maximum containment. This cabinet is completely sealed and is used when working with highly infectious or hazardous materials. Personnel access is provided through attached gloves.  

The Biosafety cabinet serve several vital functions in the laboratory, such:

Personnel Protection:

Biosafety Cabinet creates a barrier between the laboratory worker and the hazardous materials, ensuring that no contaminants escape into the environment. Airflow within the cabinet is directed away from the operator.

Product Protection:

In addition to protecting personnel, Biosafety cabinet safeguard the experiments and materials being worked on from contamination. This is crucial when handling sensitive or valuable samples.

Microbiological Containment:

Biosafety Cabinet maintains a sterile work environment by creating a laminar flow of HEPA-filtered air that sweeps contaminants away from the work surface and towards the exhaust system.

Environmental Protection:

Biosafety cabinet prevents the release of potentially harmful biological agents into the laboratory or surrounding areas. Air inside the cabinet is filtered before being exhausted to the environment.    

The Proper Use & Maintenance of Biosafety Cabinet, related to ensure the effectiveness of biosafety cabinets, regular maintenance and proper usage are essential.  

There are some key maintenance and usage tips:

Personal Protective Equipment (PPE):

Always wear appropriate PPE, including lab coats, gloves, and safety goggles when working in a BSC.

Proper Setup:

Position the cabinet away from doors, windows, and high-traffic areas to avoid disruptions in airflow. Follow manufacturer guidelines for installation.

Work Techniques:

Follow proper aseptic techniques when working inside the cabinet. Minimize movements and activities that disrupt the airflow.


Clean and disinfect the cabinet regularly to prevent contamination buildup. Ensure that all materials and equipment are compatible with the disinfectants used.


BSCs should be certified annually by qualified professionals to verify their performance and safety. Certification ensures that the cabinet meets the required standards.  

As Conclusion, The Biosafety cabinet is indispensable tools in Laboratories that handle hazardous biological materials. They provide a secure and controlled environment for working with biological agents, safeguarding laboratory personnel, experiments, and the environment. To understanding the different types of biosafety cabinets, their functions, and adhering to maintenance and usage guidelines are essential for ensuring safety and success in Laboratory research and diagnostic. (IW 0910).

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